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Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye

  • Ewa M. CzekanskaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 740)

Abstract

The Alamar Blue assay is based on enzymatic reduction of indicator dye by viable cells and serves as an effective tool for assessing cell proliferation and as a screening technique. It can be applied in studies concentrating on animal, plant, yeast, and bacteria cells. Among the various methods for cell viability and cytotoxicity, it utilises all features of ideal and reliable test; it is one-step, sensitive, safe, non-toxic for cells, and cost-effective.

Key words

Cell proliferation Resazurin Alamar Blue Metabolic activity Oxidoreductases 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.AO Research InstituteDavos PlatzSwitzerland

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