Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods

  • Kristine S. Louis
  • Andre C. SiegelEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 740)


One of the traditional methods of cell viability analysis is the use of trypan blue dye exclusion staining. This technique has been the standard methodology used in academic research laboratories and industrial biotechnology plants. Cells were routinely counted manually with a hemocytometer. In recent years, modern automated instrumentation has been introduced to supplement this traditional technique with the efficiency and reproducibility of computer control, advanced imaging, and automated sample handling.

Key words

Cell viability Cell counting Trypan blue Hemocytometer Automation 


  1. 1.
    Cell and Tissue Culture: Laboratory Procedures. (1995) (Doyle, A., Griffiths, J.B., and Newell, D.G., Ed.). John Wiley & Sons, Inc., Chichester, England.Google Scholar
  2. 2.
    Beckman Coulter, Inc. (2004) Vi-CELL™ XR Cell Viability Analyzer: Reference Manual. 1-82.Google Scholar
  3. 3.
    Nexcelom Bioscience. (2008) Cellometer® Vision: Automatic Cell Counter with Fluorescence Detection.Google Scholar
  4. 4.
    Invitrogen. (2008) Countess™ Automated Cell Counter.Google Scholar
  5. 5.
  6. 6.
    Louis, K.S., Siegel, A.C., and Levy, G.A. (2007) Comparison of Manual versus Automated Trypan Blue Dye Exclusion Method for Cell Counting. Web site:

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.MaRS Centre, Toronto MedicalUniversity Health NetworkTorontoCanada

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