Atomic Force Microscopy in Biomedical Research pp 211-221

Part of the Methods in Molecular Biology book series (MIMB, volume 736) | Cite as

Atomic Force Microscopy as a Tool for the Study of the Ultrastructure of Trypanosomatid Parasites

  • Wanderley de Souza
  • Gustavo M. Rocha
  • Kildare Miranda
  • Paulo M. Bisch
  • Gilberto Weissmuller


Here, we describe the methodology currently used to analyze the structural organization of protozoa of the Trypanosomatidae family by atomic force microscopy. The results are compared with those obtained using light, scanning, and transmission electron microscopy.

Key words

Parasitic protozoa Trypanosomatids Atomic force microscopy Scanning electron microscopy 


  1. 1.
    Binnig, G. and Rohrer, H. (1982) Scanning tunneling microscopy. Helv. Phys. Acta. 55, 726–735.Google Scholar
  2. 2.
    Binnig, G., Quate, C. F. and Gerber, C. (1986) Atomic force microscopy. Phys. Rev. Lett. 56, 930–933.Google Scholar
  3. 3.
    Bustamante, C. and Dunlap, D. (1991) Application of scanning tunneling microscopy to structural biology. Semin. Cell Biol. 2, 179–185.Google Scholar
  4. 4.
    Dvorak, J. A., Kobayashi, S., Abe, K., Fujiwara, T., Takeuchi, T. and Nagao, E. (2000) The application of the atomic force microscope to studies of medically important protozoan parasites. J. Elec. Microsc. 49, 429–435.Google Scholar
  5. 5.
    Akaki, M., Nakano, Y., Nagayasu, E., Nagakura, K., Kawai, S. and Aikawa, M. (2001) Invasive forms of Toxoplasma gondii, Leishmania amazonensis and Trypanosoma cruzi have a positive charge at their contact site with host cells. Parasitol. Res. 87, 193–197.Google Scholar
  6. 6.
    Dufrêne, Y. F. (2002) Atomic force microscopy, a powerful tool in microbiology. J. Bacteriol. 184, 5205–5213.Google Scholar
  7. 7.
    Dufrêne, Y. F. (2008) Towards nanomicrobiology using atomic force microscopy. Nat. Ver. Microbiol. 6, 674–680.Google Scholar
  8. 8.
    Wright, C. J. and Armstrong, I. (2006) The application of atomic force microscopy force measurements to the characterization of microbial surfaces. Surf. Interface Anal. 38, 1419–1428.Google Scholar
  9. 9.
    Garcia, C. R., Takeuschi, M., Yoshioka, K. and Miyamoto, H. (1997) Imaging Plasmodium falciparum-infected ghost and parasite by atomic force microscopy. J. Struct. Biol. 119, 92–98.Google Scholar
  10. 10.
    Rocha, G. M., Miranda, K., Weissmüller, G., Bisch, P. M., de Souza, W. (2008) Ultrastructure of Trypanosoma cruzi revisited by atomic force microscopy. Microsc. Res. Tech. 71, 133–139.Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Wanderley de Souza
    • 1
    • 2
  • Gustavo M. Rocha
  • Kildare Miranda
  • Paulo M. Bisch
  • Gilberto Weissmuller
  1. 1.Instituto de Biofísica Carlos Chagas FilhoUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Diretoria de ProgramasNacional de Metrologia, Normalização e Qualidade Industrial – INMETRORio de JaneiroBrazil

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