Protein Microarray for Disease Analysis pp 81-104

Part of the Methods in Molecular Biology book series (MIMB, volume 723)

Recombinant Antigen Microarrays for Serum/Plasma Antibody Detection

Protocol

Abstract

Recombinant antigen arrays represent a new frontier in parallel analysis of multiple immune response profiles requiring only minute blood samples. In this article, we review the benefits and pitfalls of recombinant antigen microarrays developed for multiplexed antibody quantification. In particular, we describe the development of antigen arrays presenting a set of Y chromosome-encoded antigens, called H-Y antigens. These H-Y antigens are immunologically recognized as minor histocompatibility antigens (mHA) following allogeneic blood and organ transplantation. Clinically relevant B-cell responses against H-Y antigens have been demonstrated in male patients receiving female hematopoietic stem cell grafts and are associated with chronic graft versus host development. This chapter discusses our recombinant antigen microarray methods to measure these clinically relevant allo-antibodies.

Key words

H-Y proteins Antibodies Plasma Recombinant antigen microarrays Minor histocompatibility antigens 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Persis P. Wadia
    • 1
  • Bita Sahaf
    • 1
  • David B. Miklos
    • 1
  1. 1.Department of Medicine, Blood and Marrow Transplantation DivisionStanford UniversityStanfordUSA

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