Label-Free Detection with Surface Plasmon Resonance Imaging
Sensors based on surface plasmon resonance have demonstrated, over the last 2 decades, to be an effective method of studying biomolecular interactions without the need for labeling. Recently, it has been adapted to high-throughput use for imaging microarray binding in real time. This provides a promising platform – a label-free protein microarray system – for the study of disease. In this example, antibody microarrays are used to efficiently profile the secretion of proteins from a cell line exposed to varying concentrations of a toxic compound.
Key wordsSurface plasmon resonance Label-free microarray Molecular interactions Protein binding Protein profiling Kinetics analysis Affinity Refractometry
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