Protein Microarray for Disease Analysis pp 15-28

Part of the Methods in Molecular Biology book series (MIMB, volume 723)

Analysis of Serum Protein Glycosylation with Antibody–Lectin Microarray for High-Throughput Biomarker Screening

Protocol

Abstract

The complexity of carbohydrate structures and their derivatives makes the study of the glycome a challenging subset of proteomic research. The microarray platform has become an essential tool to characterize glycan structure and to study glycosylation-related biological interactions, by using probes as a means to interrogate the spotted or captured glycosylated molecules on the arrays. The high-throughput and reproducible nature of microarray platforms have been highlighted by their extensive applications in the field of biomarker validation, where a large number of samples must be analyzed multiple times. This chapter presents an antibody–lectin microarray approach, which allows the efficient, multiplexed study of the glycosylation of multiple individual proteins from complex mixtures with both fluorescence labeling detection and label-free detection based on mass spectrometry.

Key words

Microarray Antibody Glycoprotein Biomarker Serum Lectin MALDI Mass spectrometry 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of ChemistryThe University of MichiganAnn ArborUSA
  2. 2.Department of Chemistry, Comprehensive Cancer CenterThe University of MichiganAnn ArborUSA
  3. 3.Department of SurgeryThe University of Michigan Medical CenterAnn ArborUSA

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