Polyamines pp 81-111 | Cite as

Identification, Chemical Synthesis, and Biological Functions of Unusual Polyamines Produced by Extreme Thermophiles

  • Tairo Oshima
  • Toshiyuki Moriya
  • Yusuke Terui
Part of the Methods in Molecular Biology book series (MIMB, volume 720)


Unusual long polyamines such as caldopentamine and caldohexamine, and branched polyamines such as tetrakis(3-aminopropyl)ammonium and N 4-aminopropylspermidine were often found in cells of extreme thermophiles and hyperthermophiles belonging to both Bacteria and Archaea domains. Some of these unusual polyamines are essential for life at extreme temperatures. In some cases, the unusual polyamines also exist in cells of nonthermophilic organisms and play important physiological roles under normal conditions. Methods for chromatographic analysis, isolation, and chemical syntheses of unusual polyamines as well as experimental methods for measuring their physiological roles are discussed. Especially, many newly improved methods for chemical syntheses are presented in this article.

Key words

Pentamine Hexamine Branched polyamine Quaternary polyamine Thermophiles Protein synthesis Depurination Reverse genetics 



Studies cited in this article were done by many former colleagues in our laboratories in University of Tokyo, Mitsubishi-Kasei Institute of Life Sciences, Tokyo Institute of Technology, Tokyo University of Pharmacy and Life Science, and the Institute of Environmental Microbiology, Kyowa-kako Company. Especially the authors are grateful to Drs. Mio Ohnuma, Taketoshi Uzawa, Yoshiko Ohno-Iwashita, and Nobuko Hamasaki-Katagiri.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Tairo Oshima
    • 1
  • Toshiyuki Moriya
    • 1
    • 2
  • Yusuke Terui
    • 3
  1. 1.Institute of Environmental MicrobiologyKyowa-kako Co.MachidaJapan
  2. 2.Department of Molecular BiologyTokyo University of Pharmacy and Life ScienceHachiojiJapan
  3. 3.Faculty of PharmacyChiba Institute of ScienceChoshi, ChibaJapan

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