Regulatory T Cells pp 45-54

Part of the Methods in Molecular Biology book series (MIMB, volume 707)

In Vitro and In Vivo Analyses of Regulatory T Cell Suppression of CD8+ T Cells



The study of regulatory T cells (Treg) requires methods for both in vivo and in vitro analyses, both of which have different limitations, but which complement each other to give a more complete picture of physiological function than either method alone. Our analyses have focused on Treg-mediated suppression of CD8+ T cells, and in particular Tregs induced by viral infection. One of the unique characteristics of virus-induced Tregs is that they can suppress CD8+ T cell function in vitro without the requirement for additional stimulation. This ability correlates with their suppressive capacity and activated status in vivo. Interestingly, while virus-induced Tregs suppress CD8+ T cell function in vitro and in vivo, they do not suppress proliferation unless they are further activated in vitro.

Key words

Regulatory T cells CD8+ T cells 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Laboratory of Persistent Viral Diseases, Rocky Mountain LaboratoriesNational Institute of Allergy and Infectious Diseases, National Institutes of HealthHamiltonUSA

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