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Methods for the Purification and Characterization of Human Adipose-Derived Stem Cells

  • Stan GronthosEmail author
  • Andrew C. W. Zannettino
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 702)

Abstract

Peripheral adipose tissue contains a population of clonogenic precursor cells referred to as adipose-derived stem cells (ASC) that retain the capacity to differentiate into multiple cell types including osteoblasts, adipocytes, chondrocytes, myocytes, and neuronal cells following ex vivo expansion. Recent studies have demonstrated that ASC are most likely derived from a perivascular niche within highly vascularised fat tissue, analogous to different mesenchymal cell populations identified in other tissues throughout the body. The following chapter describes techniques to prospectively isolate clonogenic ASC from adult human adipose tissue using antibodies directed against perivascular markers and methods to immunophenotypically characterize their ex vivo expanded progeny.

Key words

Bone marrow stromal stem cells Mesenchymal stem cells Magnetic-activated cell ­sorting Fluorescence-activated cell sorting 

Notes

Acknowledgments

The authors wish to acknowledge the technical assistance of Ms. Sharon Paton and Ms. Romana Borowicz.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Mesenchymal Stem Cell Group, Department of Haematology, Institute of Medical and Veterinary Science/Hanson Institute and Centre for Stem Cell ResearchUniversity of AdelaideAdelaideAustralia
  2. 2.Myeloma Research Laboratory, Department of Haematology, Institute of Medical and Veterinary Science/Centre for Cancer Biology and the Centre for Stem Cell ResearchUniversity of AdelaideAdelaideAustralia

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