Adipose-Derived Stem Cells pp 193-200

Part of the Methods in Molecular Biology book series (MIMB, volume 702)

Adipogenic Differentiation of Adipose-Derived Stem Cells

  • Gang Yu
  • Z. Elizabeth Floyd
  • Xiying Wu
  • Teddi Hebert
  • Yuan-Di C. Halvorsen
  • Benjamin M. Buehrer
  • Jeffrey M. Gimble
Protocol

Abstract

The primary physiological function of adipose-derived stem cells (ASCs) is to differentiate into adipose tissue. It is now possible to isolate, expand, and cryopreserve ASC from adipose depots of many animal species. These ASC can be induced to undergo adipogenic differentiation in vitro by exposure to a cocktail of chemical agents or inductive growth factors. The current chapter describes methods to induce adipogenesis and to quantify this differentiation process in vitro.

Key words

Adipose-derived stem cells Adipogenesis Basic fibroblast growth factor Bone morphogenetic protein CCAAT/enhancer binding protein α Cyclic adenosine monophosphate Epidermal growth factor Glucocorticoid receptor Indomethacin Insulin Isobutylmethylxanthine Peroxisome proliferator-activated receptor γ2 Rabbit serum 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Gang Yu
    • 1
  • Z. Elizabeth Floyd
    • 2
  • Xiying Wu
    • 1
  • Teddi Hebert
    • 1
  • Yuan-Di C. Halvorsen
    • 3
  • Benjamin M. Buehrer
    • 4
  • Jeffrey M. Gimble
    • 1
  1. 1.Stem Cell Biology Laboratory, Pennington Biomedical Research CenterLouisiana State University SystemBaton RougeUSA
  2. 2.Ubiquitin Laboratory, Pennington Biomedical Research CenterLouisiana State University SystemBaton RougeUSA
  3. 3.Center for Computational and Integrative BiologyHarvard Medical SchoolBostonUSA
  4. 4.ZenBio, Inc., ResearchTriangle ParkUSA

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