Flow Cytometry Protocols pp 449-469

Part of the Methods in Molecular Biology book series (MIMB, volume 699)

Standard Practice for Cell Sorting in a BSL-3 Facility

  • Stephen P. Perfetto
  • David R. Ambrozak
  • Richard Nguyen
  • Mario Roederer
  • Richard A. Koup
  • Kevin L. Holmes
Protocol

Abstract

Over the past decade, there has been a rapid growth in the number of BSL-3 and BSL-4 laboratories in the USA and an increase in demand for infectious cell sorting in BSL-3 laboratories. In 2007, the International Society for Advancement of Cytometry (ISAC) Biosafety Committee published standards for the sorting of unfixed cells and is an important resource for biosafety procedures when performing infectious cell sorting. Following a careful risk assessment, if it is determined that a cell sorter must be located within a BSL-3 laboratory, there are a variety of factors to be considered prior to the establishment of the laboratory. This chapter outlines procedures for infectious cell sorting in a BSL-3 environment to facilitate the establishment and safe operation of a BSL-3 cell sorting laboratory. Subjects covered include containment verification, remote operation, disinfection, personal protective equipment (PPE), and instrument-specific modifications for enhanced aerosol evacuation.

Key words

Flow cytometry High speed sorting Biosafety Biohazard Safety in flow cytometry 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Stephen P. Perfetto
    • 1
  • David R. Ambrozak
    • 1
  • Richard Nguyen
    • 1
  • Mario Roederer
    • 1
  • Richard A. Koup
    • 1
  • Kevin L. Holmes
    • 2
  1. 1.Vaccine Research Center, National Institute of Allergy and Infectious DiseasesNational Institutes of HealthBethesdaUSA
  2. 2.Flow Cytometry Section, Research Technologies Branch, National Institute of Allergy and Infectious DiseasesNational Institutes of HealthBethesdaUSA

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