Isolation and Growth of Adipose Tissue-Derived Stem Cells

  • Vladimir Zachar
  • Jeppe Grøndahl Rasmussen
  • Trine Fink
Part of the Methods in Molecular Biology book series (MIMB, volume 698)


Fat tissue provides a rich and easily accessible supply of stem cells that feature the general differentiation potential and plasticity of mesenchymal stem cells. These stem cells, variably designated adipose-derived stem cells (ASCs), hold a great promise for regeneration in vivo and tissue engineering. In order to be able to obtain ASC preparations suitable for basic investigations as well as development of future therapeutic protocols, it is important that the critical isolation steps are properly carried out. Here, we describe in detail enzyme-based procedure for isolation and propagation of ASCs from the human subcutaneous fat tissue that is also adoptable to several animal species. The critical steps and impact of possible modifications on the final outcome are discussed, and useful hints are provided to streamline the troubleshooting.

Key words

Adipose stem cells Isolation Growth Fat tissue Collagenase Hypoxia 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Vladimir Zachar
    • 1
  • Jeppe Grøndahl Rasmussen
  • Trine Fink
  1. 1.Laboratory for Stem Cell ResearchAalborg UniversityAalborgDenmark

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