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Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells: Tips and Tricks

  • Luis A. Solchaga
  • Kitsie J. Penick
  • Jean F. Welter
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 698)

Abstract

It is well known that adult cartilage lacks the ability to repair itself; this makes articular cartilage a very attractive target for tissue engineering. The majority of articular cartilage repair models attempt to deliver or recruit reparative cells to the site of injury. A number of efforts are directed to the characterization of progenitor cells and the understanding of the mechanisms involved in their chondrogenic differentiation. Our laboratory has focused on cartilage repair using mesenchymal stem cells and studied their differentiation into cartilage. Mesenchymal stem cells are attractive candidates for cartilage repair due to their osteogenic and chondrogenic potential, ease of harvest, and ease of expansion in culture. However, the need for chondrogenic differentiation is superposed on other technical issues associated with cartilage repair; this adds a level of complexity over using mature chondrocytes. This chapter will focus on the methods involved in the isolation and expansion of human mesenchymal stem cells, their differentiation along the chondrogenic lineage, and the qualitative and quantitative assessment of chondrogenic differentiation.

Key words

Mesenchymal stem cell Chondrogenesis Tissue culture Fibroblast growth factor-2 Transforming growth factor-beta Aggregate culture Cartilage 

Notes

Acknowledgments

This work was supported by grants from the Arthritis Foundation (PIs Jean F. Welter and Luis A. Solchaga), the Ohio Department of Development (PI Luis A. Solchaga), NIH; R01 AR050208 (PI Jean F. Welter) and P01 AR053622 (PIs Jean F. Welter and Luis A. Solchaga); and the Hematopoietic Stem Cell Core Facility of the Case Comprehensive Cancer Center (P30 CA43703; PI Stanton L. Gerson). The authors also want to thank Ms. Harris who processes the majority of the bone marrow specimens used in the laboratory.

References

  1. 1.
    Minguell JJ, Erices A, Conget P. Mesenchymal stem cells. Exp Biol Med (Maywood). 2001;226(6):507–20.Google Scholar
  2. 2.
    Friedenstein AJ. Precursor cells of mechanocytes. Int Rev Cytol. 1976;47:327–59.PubMedCrossRefGoogle Scholar
  3. 3.
    Owen M. Marrow stromal stem cells. J Cell Sci Suppl. 1988;10:63–76.PubMedGoogle Scholar
  4. 4.
    Owen M, Friedenstein AJ. Stromal stem cells: marrow-derived osteogenic precursors. Ciba Found Symp. 1988;136:42–60.PubMedGoogle Scholar
  5. 5.
    Caplan AI. The mesengenic process. Clin Plast Surg. 1994;21(3):429–35.PubMedGoogle Scholar
  6. 6.
    Ohgushi H, Goldberg VM, Caplan AI. Heterotopic osteogenesis in porous ceramics induced by marrow cells. J Orthop Res. 1989;7(4):568–78.PubMedCrossRefGoogle Scholar
  7. 7.
    Ohgushi H, Okumura M. Osteogenic capacity of rat and human marrow cells in porous ceramics. Experiments in athymic (nude) mice. Acta Orthop Scand. 1990;61(5):431–4.PubMedCrossRefGoogle Scholar
  8. 8.
    Ohgushi H, Okumura M, Tamai S, Shors EC, Caplan AI. Marrow cell induced osteogenesis in porous hydroxyapatite and tricalcium phosphate: a comparative histomorphometric study of ectopic bone formation. J Biomed Mater Res. 1990;24(12):1563–70.PubMedCrossRefGoogle Scholar
  9. 9.
    Goshima J, Goldberg VM, Caplan AI. The osteogenic potential of culture-expanded rat marrow mesenchymal cells assayed in vivo in calcium phosphate ceramic blocks. Clin Orthop Relat Res. 1991(262):298–311.PubMedGoogle Scholar
  10. 10.
    Goshima J, Goldberg VM, Caplan AI. Osteogenic potential of culture-expanded rat marrow cells as assayed in vivo with porous calcium phosphate ceramic. Biomaterials. 1991;12(2):253–8.PubMedCrossRefGoogle Scholar
  11. 11.
    Haynesworth SE, Goshima J, Goldberg VM, Caplan AI. Characterization of cells with osteogenic potential from human bone marrow. Bone. 1992;13:81–8.PubMedCrossRefGoogle Scholar
  12. 12.
    Lennon DP, Haynesworth SE, Bruder SP, Jaiswal N, Caplan AI. Human and animal mesenchymal progenitor cells from bone marrow: Identification of serum for optimal selection and proliferation. In Vitro Cell Dev Biol. 1996;32(10):602–11.CrossRefGoogle Scholar
  13. 13.
    Bruder SP, Jaiswal N, Haynesworth SE. Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation. J Cell Biochem. 1997;64(2):278–94.PubMedCrossRefGoogle Scholar
  14. 14.
    Digirolamo CM, Stokes D, Colter D, Phinney DG, Class R, Prockop DJ. Propagation and senescence of human marrow stromal cells in culture: a simple colony-forming assay identifies samples with the greatest potential to propagate and differentiate. Br J Haematol. 1999;107(2):275–81.PubMedCrossRefGoogle Scholar
  15. 15.
    Phinney DG, Kopen G, Righter W, Webster S, Tremain N, Prockop DJ. Donor variation in the growth properties and osteogenic potential of human marrow stromal cells. J Cell Biochem. 1999;75(3):424–36.PubMedCrossRefGoogle Scholar
  16. 16.
    Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284(5411):143–7.PubMedCrossRefGoogle Scholar
  17. 17.
    Jaiswal N, Haynesworth SE, Caplan AI, Bruder SP. Osteogenic differentiation of purified culture-expanded human mesenchymal stem cells in vitro. J Cell Biochem. 1997;64:295–312.PubMedCrossRefGoogle Scholar
  18. 18.
    Johnstone B, Hering TM, Caplan AI, Goldberg VM, Yoo JU. In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells. Exp Cell Res. 1998;238(1):265–72.PubMedCrossRefGoogle Scholar
  19. 19.
    Yoo JU, Barthel TS, Nishimura K, Solchaga L, Caplan AI, Goldberg VM, et al. The chondrogenic potential of human bone-marrow-derived mesenchymal progenitor cells. J Bone Joint Surg Am. 1998;80(12):1745–57.PubMedGoogle Scholar
  20. 20.
    Lee SH, Lumelsky N, Studer L, Auerbach JM, McKay RD. Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells. Nat Biotechnol. 2000;18(6):675–9.PubMedCrossRefGoogle Scholar
  21. 21.
    Mackay AM, Beck SC, Murphy JM, Barry FP, Chichester CO, Pittenger MF. Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow. Tissue Eng. 1998;4(4):415–28.PubMedCrossRefGoogle Scholar
  22. 22.
    Pittenger MF, Mbalaviele G, Black M, Mosca JD, Marshak DR. Mesenchymal stem cells. In: Koller MR, Palsson BO, Masters JRW, editors. Primary mesenchymal cells. Dordrech: Kluwer Academic Publishers; 2001. p. 189–207.Google Scholar
  23. 23.
    Banfi A, Bianchi G, Notaro R, Luzzatto L, Cancedda R, Quarto R. Replicative aging and gene expression in long-term cultures of human bone marrow stromal cells. Tissue Eng. 2002;8(6):901–10.PubMedCrossRefGoogle Scholar
  24. 24.
    Langer R, Vacanti JP. Tissue engineering. Science. 1993;260(5110):920–6.PubMedCrossRefGoogle Scholar
  25. 25.
    Vacanti CA, Vacanti JP. The science of tissue engineering. Orthop Clin North Am. 2000;31(3):351–6.PubMedCrossRefGoogle Scholar
  26. 26.
    Vacanti J, Langer R. Tissue engineering: the design and fabrication of living replacement devices for surgical re-construction and transplantation. Lancet. 1999;354(Suppl. 1):SI32–SI4.PubMedGoogle Scholar
  27. 27.
    Bonassar LJ, Vacanti CA. Tissue engineering: the first decade and beyond. J Cell Biochem Suppl. 1998;30–31:297–303.PubMedCrossRefGoogle Scholar
  28. 28.
    Pearson RG, Bhandari R, Quirk RA, Shakesheff KM. Recent advances in tissue engineering: an invited review. J Long Term Eff Med Implants. 2002;12(1):1–33.PubMedGoogle Scholar
  29. 29.
    Temenoff JS, Mikos AG. Review: tissue engineering for regeneration of articular cartilage. Biomaterials. 2000;21(5):431–40.PubMedCrossRefGoogle Scholar
  30. 30.
    O’Driscoll SW. Preclinical cartilage repair: current status and future perspectives. Clin Orthop. 2001(391 Suppl):S397–401.PubMedCrossRefGoogle Scholar
  31. 31.
    Luyten FP, Dell’Accio F, De Bari C. Skeletal tissue engineering: opportunities and challenges. Best Pract Res Clin Rheumatol. 2001;15(5):759–69.PubMedCrossRefGoogle Scholar
  32. 32.
    Musgrave DS, Fu FH, Huard J. Gene therapy and tissue engineering in orthopaedic surgery. J Am Acad Orthop Surg. 2002;10(1):6–15.PubMedGoogle Scholar
  33. 33.
    Risbud M. Tissue engineering: implications in the treatment of organ and tissue defects. Biogerontology. 2001;2(2):117–25.PubMedCrossRefGoogle Scholar
  34. 34.
    Guilak F, Butler DL, Goldstein SA. Functional tissue engineering: the role of biomechanics in articular cartilage repair. Clin Orthop. 2001(391 Suppl):S295–305.PubMedCrossRefGoogle Scholar
  35. 35.
    Risbud MV, Sittinger M. Tissue engineering: advances in in vitro cartilage generation. Trends Biotechnol. 2002;20(8):351–6.PubMedCrossRefGoogle Scholar
  36. 36.
    Caplan AI, Goldberg VM. Principles of tissue engineered regeneration of skeletal tissues. Clin Orthop Relat Res. 1999(367 Suppl):S12–6.PubMedCrossRefGoogle Scholar
  37. 37.
    Caplan AI, Elyaderani M, Mochizuki Y, Wakitani S, Goldberg VM. Principles of cartilage repair and regeneration. Clin Orthop Relat Res. 1997;342:254–69.PubMedCrossRefGoogle Scholar
  38. 38.
    Hunter W. Of the structure and diseases of articulating cartilages. Philos Trans. 1743;42:514–21.Google Scholar
  39. 39.
    Buckwalter J. Articular cartilage: injuries and potential for healing. J Orthop Sports Phys Ther. 1998;28:192–202.PubMedGoogle Scholar
  40. 40.
    Buckwalter JA, Mankin HJ. Articular cartilage repair and transplantation. Arthritis Rheum. 1998;41(8):1331–42.PubMedCrossRefGoogle Scholar
  41. 41.
    Buckwalter JA, Mankin HJ. Articular cartilage: degeneration and osteoarthritis, repair, regeneration, and transplantation. Instr Course Lect. 1998;47:487–504.PubMedGoogle Scholar
  42. 42.
    Mankin HJ. The reaction of articular cartilage to injury and osteoarthritis (first of two parts). N Engl J Med. 1974;291(24):1285–92.PubMedCrossRefGoogle Scholar
  43. 43.
    Mankin HJ. The reaction of articular cartilage to injury and osteoarthritis (second of two parts). N Engl J Med. 1974;291(25):1335–40.PubMedCrossRefGoogle Scholar
  44. 44.
    Mankin HJ, Buckwalter JA. Restoration of the osteoarthrotic joint [editorial]. J Bone Joint Surg Am. 1996;78(1):1–2.PubMedGoogle Scholar
  45. 45.
    Chesterman PJ, Smith AU. Homotrans­plantation of articular cartilage and isolated chondrocytes. An experimental study in rabbits. J Bone Joint Surg Br. 1968;50(1):184–97.PubMedGoogle Scholar
  46. 46.
    Bentley G, Greer RB 3rd. Homotransplantation of isolated epiphyseal and articular cartilage chondrocytes into joint surfaces of rabbits. Nature. 1971;230(5293):385–8.PubMedCrossRefGoogle Scholar
  47. 47.
    Wakitani S, Kimura T, Hirooka A, Ochi T, Yoneda M, Yasui N, et al. Repair of rabbit articular surfaces with allograft chondrocytes embedded in collagen gel. J Bone Joint Surg Br. 1989;71(1):74–80.PubMedGoogle Scholar
  48. 48.
    Vacanti CA, Kim W, Schloo B, Upton J, Vacanti JP. Joint resurfacing with cartilage grown in situ from cell-polymer structures. Am J Sports Med. 1994;22(4):485–8.PubMedCrossRefGoogle Scholar
  49. 49.
    Freed LE, Grande DA, Lingbin Z, Emmanual J, Marquis JC, Langer R. Joint resurfacing using allograft chondrocytes and synthetic biodegradable polymer scaffolds. J Biomed Mater Res. 1994;28(8):891–9.PubMedCrossRefGoogle Scholar
  50. 50.
    Frenkel SR, Toolan B, Menche D, Pitman MI, Pachence JM. Chondrocyte transplantation using a collagen bilayer matrix for cartilage repair. J Bone Joint Surg Br. 1997;79(5):831–6.PubMedCrossRefGoogle Scholar
  51. 51.
    Nehrer S, Breinan HA, Ramappa A, Shortkroff S, Young G, Minas T, et al. Canine chondrocytes seeded in type I and type II collagen implants investigated in vitro. J Biomed Mater Res. 1997;38(2):95–104.PubMedCrossRefGoogle Scholar
  52. 52.
    Breinan HA, Minas T, Hsu HP, Nehrer S, Sledge CB, Spector M. Effect of cultured autologous chondrocytes on repair of chondral defects in a canine model. J Bone Joint Surg Am. 1997;79(10):1439–51.PubMedGoogle Scholar
  53. 53.
    Minas T. Chondrocyte implantation in the repair of chondral lesions of the knee: economics and quality of life. Am J Orthop. 1998;27(11):739–44.PubMedGoogle Scholar
  54. 54.
    Giannini S, Buda R, Grigolo B, Vannini F. Autologous chondrocyte transplantation in osteochondral lesions of the ankle joint. Foot Ankle Int. 2001;22(6):513–7.PubMedGoogle Scholar
  55. 55.
    Breinan HA, Minas T, Hsu HP, Nehrer S, Shortkroff S, Spector M. Autologous chondrocyte implantation in a canine model: change in composition of reparative tissue with time. J Orthop Res. 2001;19(3):482–92.PubMedCrossRefGoogle Scholar
  56. 56.
    Brittberg M, Lindahl A, Nilsson A, Ohlsson C, Isaksson O, Peterson L. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation [see comments]. N Engl J Med. 1994;331(14):889–95.PubMedCrossRefGoogle Scholar
  57. 57.
    Amiel D, Coutts RD, Abel M, Stewart W, Harwood F, Akeson WH. Rib perichondrial grafts for the repair of full-thickness articular-cartilage defects. A morphological and biochemical study in rabbits. J Bone Joint Surg Am. 1985;67(6):911–20.PubMedGoogle Scholar
  58. 58.
    O’Driscoll SW, Keeley FW, Salter RB. The chondrogenic potential of free autogenous periosteal grafts for biological resurfacing of major full-thickness defects in joint surfaces under the influence of continuous passive motion. An experimental investigation in the rabbit. J Bone Joint Surg Am. 1986;68(7):1017–35.PubMedGoogle Scholar
  59. 59.
    Amiel D, Coutts RD, Harwood FL, Ishizue KK, Kleiner JB. The chondrogenesis of rib perichondrial grafts for repair of full thickness articular cartilage defects in a rabbit model: a one year postoperative assessment. Connect Tissue Res. 1988;18(1):27–39.PubMedCrossRefGoogle Scholar
  60. 60.
    Shahgaldi BF, Amis AA, Heatley FW, McDowell J, Bentley G. Repair of cartilage lesions using biological implants. A comparative histological and biomechanical study in goats. Journal of Bone & Joint Surgery - British Volume. 1991;73(1):57-64.PubMedGoogle Scholar
  61. 61.
    Wakitani S, Goto T, Pineda SJ, Young RG, Mansour JM, Caplan AI, et al. Mesenchymal cell-based repair of large, full-thickness defects of articular cartilage. J Bone Joint Surg Am. 1994;76(4):579–92.PubMedGoogle Scholar
  62. 62.
    Chu CR, Coutts RD, Yoshioka M, Harwood FL, Monosov AZ, Amiel D. Articular cartilage repair using allogeneic perichondrocyte-seeded biodegradable porous polylactic acid (PLA): a tissue-engineering study. J Biomed Mater Res. 1995;29(9):1147–54.PubMedCrossRefGoogle Scholar
  63. 63.
    Butnariu-Ephrat M, Robinson D, Mendes DG, Halperin N, Nevo Z. Resurfacing of goat articular cartilage by chondrocytes derived from bone marrow. Clin Orthop Relat Res. 1996;330:234–43.PubMedCrossRefGoogle Scholar
  64. 64.
    Hunziker EB, Rosenberg LC. Repair of partial-thickness defects in articular cartilage: cell recruitment from the synovial membrane. J Bone Joint Surg Am. 1996;78(5):721–33.PubMedGoogle Scholar
  65. 65.
    Chu CR, Dounchis JS, Yoshioka M, Sah RL, Coutts RD, Amiel D. Osteochondral repair using perichondrial cells. A 1-year study in rabbits. Clin Orthop Relat Res. 1997(340):220–9.PubMedCrossRefGoogle Scholar
  66. 66.
    Goldberg VM, Caplan AI. Biologic restoration of articular surfaces. Inst Course Lect. 1999;48:623–7.Google Scholar
  67. 67.
    Johnstone B, Yoo JU. Autologous mesenchymal progenitor cells in articular cartilage repair. Clin Orthop Relat Res. 1999(367 Suppl):S156–62.PubMedCrossRefGoogle Scholar
  68. 68.
    Dounchis JS, Bae WC, Chen AC, Sah RL, Coutts RD, Amiel D. Cartilage repair with autogenic perichondrium cell and polylactic acid grafts. Clin Orthop Relat Res. 2000(377):248–64.PubMedCrossRefGoogle Scholar
  69. 69.
    Solchaga LA, Gao J, Dennis JE, Awadallah A, Lundberg M, Caplan AI, et al. Treatment of osteochondral defects with autologous bone marrow in a hyaluronan-based delivery vehicle. Tissue Eng. 2002;8(2):333–47.PubMedCrossRefGoogle Scholar
  70. 70.
    Caplan AI. Mesenchymal stem cells. J Orthop Res. 1991;9(5):641–50.PubMedCrossRefGoogle Scholar
  71. 71.
    Ashton BA, Allen TD, Howlett CR, Eaglesom CC, Hattori A, Owen M. Formation of bone and cartilage by marrow stromal cells in diffusion chambers in vivo. Clin Orthop. 1980(151):294–307.PubMedGoogle Scholar
  72. 72.
    Angele P, Smith C, Mansour J, Jepsen K, Johnstone B, Yoo J. Effects of cyclic hydrostatic pressure on the chondrogenic differentiation of mesenchymal progenitor cells. Trans Orthop Res Soc. 2000;25(2):645.Google Scholar
  73. 73.
    Hanada K, Solchaga LA, Caplan AI, Hering TM, Goldberg VM, Yoo JU, et al. BMP-2 induction and TGF-beta 1 modulation of rat periosteal cell chondrogenesis. J Cell Biochem. 2001;81(2): 284–94.PubMedCrossRefGoogle Scholar
  74. 74.
    Solchaga LA, Penick K, Porter JD, Goldberg VM, Caplan AI, Welter JF. FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cells. J Cell Physiol. 2005;203(2):398–409.PubMedCrossRefGoogle Scholar
  75. 75.
    Bianchi G, Banfi A, Mastrogiacomo M, Notaro R, Luzzatto L, Cancedda R, et al. Ex vivo enrichment of mesenchymal cell progenitors by fibroblast growth factor 2. Exp Cell Res. 2003;287(1):98–105.PubMedCrossRefGoogle Scholar
  76. 76.
    Martin I, Muraglia A, Campanile G, Cancedda R, Quarto R. Fibroblast growth factor-2 supports ex vivo expansion and maintenance of osteogenic precursors from human bone marrow. Endocrinology. 1997;138(10):4456–62.PubMedCrossRefGoogle Scholar
  77. 77.
    Tsutsumi S, Shimazu A, Miyazaki K, Pan H, Koike C, Yoshida E, et al. Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF. Biochem Biophys Res Commun. 2001;288(2):413–9.PubMedCrossRefGoogle Scholar
  78. 78.
    Ballock RT, Reddi AH. Thyroxine is the serum factor that regulates morphogenesis of columnar cartilage from isolated chondrocytes in chemically defined medium. J Cell Biol. 1994;126(5):1311–8.PubMedCrossRefGoogle Scholar
  79. 79.
    Penick KJ, Solchaga LA, Welter JF. High-throughput aggregate culture system to assess the chondrogenic potential of mesenchymal stem cells. Biotechniques. 2005;39(5):687–91.PubMedCrossRefGoogle Scholar
  80. 80.
    Ponticiello MS, Schinagl RM, Kadiyala S, Barry FP. Gelatin-based resorbable sponge as a carrier matrix for human mesenchymal stem cells in cartilage regeneration therapy. J Biomed Mater Res. 2000;52(2):246–55.PubMedCrossRefGoogle Scholar
  81. 81.
    Carrino DA, Arias JL, Caplan AI. A spectrophotometric modification of a sensitive densitometric Safranin O assay for glycosaminoglycans. Biochem Int. 1991;24(3):485–95.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Luis A. Solchaga
    • 1
  • Kitsie J. Penick
  • Jean F. Welter
  1. 1.Case Comprehensive Cancer CenterCase Western Reserve UniversityClevelandUSA

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