Adipogenic Differentiation of Human Mesenchymal Stem Cells

  • Trine Fink
  • Vladimir Zachar
Part of the Methods in Molecular Biology book series (MIMB, volume 698)


Mesenchymal stem cells have the capability to differentiate into a number of cell types including adipocytes. The adipocytic phenotype is characterized by intracellular accumulation of lipid droplets as well as transcription of adipocyte-specific genes. This paper details a basic protocol for adipogenic induction of bone marrow and adipose tissue-derived stem cells, as well as protocols for staining lipid accumulation and the transcriptional analysis of PPAR-γ and aP2 by real-time RT-PCR.

Key words

Mesenchymal stem cells Adipogenesis Differentiation Lipid staining PPAR-γ aP2 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Laboratory for Stem Cell ResearchAalborg UniversityAalborgDenmark

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