Osteogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells

  • Deepak M. Gupta
  • Nicholas J. Panetta
  • Michael T. Longaker
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 698)

Abstract

A comprehensive knowledge of the molecular biology underlying osteogenic differentiation in a controlled, laboratory setting may promise optimization of future cell-based tissue engineering strategies for clinical problems. The scope of this review encompasses a discussion of the methodology utilized to perform such studies. Our laboratory routinely performs both in vitro and in vivo assays underlying osteogenic differentiation, and the widespread use of singular methodology across multiple investigators and institutions promises great advancements for the skeletal tissue engineering community.

Key words

Osteogenic differentiation Skeletal tissue engineering Regeneration Mesenchymal stromal cells Bone marrow Adipose derived stromal cells Alizarin red 

Notes

Acknowledgments

This work was supported by NIH R01 DE014526, NIH R21 DE019274, and grants from the Oak and Hagey Foundations to M.T.L., NIH F32 AR055871, Stanford University Dean’s Award and Lucile Packard Children’s Health Research Program Award to D.M.G., and an American College of Surgeons Resident Research Award and Lucile Packard Children’s Health Research Program Award to N.J.P.

Reference

  1. 1.
    Cowan, C.M., Shi, Y.Y., Aalami, O.O. et al. Adipose-derived adult stromal cells heal ­critical-size mouse calvarial defects. Nat Biotechnol 22, 560–7 (2004).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Deepak M. Gupta
  • Nicholas J. Panetta
  • Michael T. Longaker
    • 1
  1. 1.Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of SurgeryStanford University School of MedicineStanfordUSA

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