Transgenesis in Mouse Embryonic Stem Cells
Traditionally, transgenic mice are generated by pronuclear injection of exogenous DNA. This technique has several limitations, including limited control over transgene expression, transgene cytotoxicity, promiscuity and silencing, and founder mouse sterility. Here we describe two protocols to generate transgenic mice from ES cell clones carrying stably integrated exogenous DNA with inducible transgene expression.
Key wordsTransgenic mice ES cells Inducible expression Cre recombinase Doxycycline