Cryopreservation of Mouse Spermatozoa and In Vitro Fertilization

  • Naomi Nakagata
Part of the Methods in Molecular Biology book series (MIMB, volume 693)


Cryopreservation of mouse spermatozoa has become the foremost technique for preserving large numbers of different strains of mice with induced mutations. Recently, we have established procedures for cryopreservation of mouse spermatozoa and in vitro fertilization using cryopreserved spermatozoa to obtain a relatively high fertilization rate. This chapter attempts to show these procedures in simple terms.

Key words

Cryopreservation Mouse sperm In vitro fertilization 


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Naomi Nakagata
    • 1
  1. 1.Division of Reproductive Engineering, Center for Animal Resources & Development (CARD)Kumamoto UniversityKumamotoJapan

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