Protocol

Light Microscopy

Volume 689 of the series Methods in Molecular Biology pp 3-18

Date:

Glycol Methacrylate Embedding for Improved Morphological, Morphometrical, and Immunohistochemical Investigations Under Light Microscopy: Testes as a Model

  • Hélio Chiarini-GarciaAffiliated withLaboratory of Structural Biology and Reproduction, Department of Morphology, ICB, Federal University of Minas Gerais Email author 
  • , Gleydes Gambogi ParreiraAffiliated withLaboratory of Structural Biology and Reproduction, Department of Morphology, ICB, Federal University of Minas Gerais
  • , Fernanda R.C.L. AlmeidaAffiliated withLaboratory of Structural Biology and Reproduction, Department of Morphology, ICB, Federal University of Minas Gerais

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Abstract

Glycol methacrylate (GMA), a water and ethanol miscible plastic resin, is a medium handy to use for light microscopy embedding that has a number of advantages than paraffin embedding. The GMA improves the histological, morphometrical, and immunohistochemical evaluations, mainly due to the accurate assessment of cytological details. This chapter focuses on our experience in the GMA processing and describes in detail the fixation, embedding, and staining methods that we have been using for testes evaluations.

Key words

Glycol methacrylate fixation embedding light microscopy testes