PCR Protocols pp 193-205 | Cite as

The Many Faces of MLPA

  • Thomas Ohnesorg
  • Erin Turbitt
  • Stefan J. White
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 687)

Abstract

Multiplex Ligation-dependent Probe Amplification (MLPA) is a PCR-based technique that was developed for identifying deletions and duplications in genomic DNA. The simplicity and sensitivity of this approach has led to it being implemented in many laboratories around the world. Since the original publication, there have been several variants of MLPA described, allowing the quantitative analysis of mRNA transcript levels, CpG methylation, complex genomic regions, and DNaseI hypersensitive sites. This chapter outlines the basic MLPA protocol, describes the different modifications and applications that have been published, and discusses the critical points during each of the steps.

Key words

MLPA Copy number variation Deletion Duplication Mutation screening Methyla­tion Expression analysis DNaseI hypersensitivity 

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Copyright information

© Humana Press 2011

Authors and Affiliations

  • Thomas Ohnesorg
    • 1
  • Erin Turbitt
    • 1
  • Stefan J. White
    • 1
  1. 1.Molecular Development, Murdoch Children’s Research InstituteRoyal Children’s HospitalMelbourneAustralia

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