Isolation and Properties of Endothelial Cells Forming the Blood-Nerve Barrier

  • Yasuteru Sano
  • Takashi Kanda
Part of the Methods in Molecular Biology book series (MIMB, volume 686)


The blood–nerve barrier (BNB) is one of the functional barriers sheltering the nervous system from circulating blood. It is very important to understand the cellular properties of endothelial cells of endoneurial origin because these cells constitute the bulk of the BNB. This chapter describes a standard protocol for isolating the endothelial cells forming the BNB. In addition, methods for confirming some of the barrier properties of isolated endothelial cells are also described.

Key words

Blood–nerve barrier Peripheral nerve microvascular endothelial cells Endoneurium Cloning Tight junction Transporter 



This work was supported in part by a Neuroimmunological Disease Research Committee grant from the Ministry of Health, Labour and Welfare, Japan and also by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture, Japan.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Yasuteru Sano
    • 1
  • Takashi Kanda
    • 1
  1. 1.Department of Neurology and Clinical NeuroscienceYamaguchi University Graduate School of MedicineUbeJapan

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