Photosynthesis Research Protocols pp 321-325 | Cite as
Rapid Isolation of Intact Chloroplasts from Spinach Leaves
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Abstract
In this chapter, a rapid method to isolate intact chloroplasts from spinach leaves is described. Intact chloroplasts are isolated using two short centrifugation steps and avoiding the use of percoll gradient. Intactness of chloroplast is evaluated by the inability of potassium ferricyanide to enter inside the chloroplasts and to act as an electron acceptor for photosystem II.
Key words
Chloroplasts Leaves Spinach Potassium ferricyanide Oxygen evolutionReferences
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