Protocol

Cell-Penetrating Peptides

Volume 683 of the series Methods in Molecular Biology pp 431-451

Date:

Intracellular Delivery of Nanoparticles with CPPs

  • Rupa SawantAffiliated withResearch Associate Center for Pharmaceutical Biotechnology and Nanomedicine, Northeastern University
  • , Vladimir TorchilinAffiliated withCenter for Pharmaceutical, Biotechnology and Nanomedicine, Northeastern University

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Abstract

Cell-penetrating peptides (CPPs), in particular TATp, have been widely used for intracellular delivery of various cargoes, both in vitro and in vivo. Modifications of nanoparticles with CPPs require either covalent or noncovalent approach. Here we describe various methods to attach CPP, such as TATp to surface of nanocarriers (such as liposomes and micelles), loading with drug or DNA and characterization of same for in vitro and in vivo applications. Due to nonselectivity of CPPs and wide distribution in nontarget areas, method for preparation of “smart” nanocarrier with hidden TATp function is also described.

Key words

CPP Liposomes Micelles Nanoparticles “Smart” Drug Delivery System TATp pH sensitive DNA