Plasmacytoid Dendritic Cells in Tolerance

  • Eric Gehrie
  • William Van der Touw
  • Jonathan S. Bromberg
  • Jordi C. OchandoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 677)


Dendritic cells (DC) are professional antigen-presenting cells (APCs) that modulate the outcome of the immune response toward immunity or tolerance. There are a large variety of DC subsets according to surface phenotype, function, and tissue distribution. Murine plasmacytoid DC (pDC) represent a distinctive DC population and are characterized by the expression of CD11c, B220, Gr-1, CD45RA, Ly49Q, BST2, and siglec-H on the cell surface. PDC act as immunogenic cell sentinels by secreting large amounts of type I interferon (IFN) in the lymph nodes in response to viral stimulation. PDC also act as tolerogenic cells when expressing the inducible tolerogenic enzyme indoleamine 2,3-dioxygenase (IDO), the inducible costimulator ligand (ICOS-L), and/or the programmed death 1 ligand (PD-L1), which mediate regulatory T-cell (Treg) development and suppression of self- and alloreactive cells. The PDC ability to induce Treg development is associated with capture and presentation of antigenic peptides associated with major histocompatibility complex (MHC) class I and II. Here, we provide the tools to study PDC development from bone marrow cultures, their antigen presentation properties, and their interactions with Treg under a tolerogenic setting of sterile inflammation.

Key words

Plasmacytoid dendritic cells Antigen presentation Regulatory T cells 



We acknowledge Dr. Tamar Hermesh for technical assistance. We also wish to acknowledge the efforts of the Mount Sinai Sorting Core Facility. This work was supported by the Programa Ramón y Cajal RYC-2006-1588, Ministerio de Educación y Ciencia SAF2007-63579, Programa José Castillejo JC2008-00065, and Programa de Investigación de Grupos Emergentes del ISCIII (to JCO), NIH R01 AI-41428, AI-72039 (to JSB), and Howard Hughes Medical Institute (to EG).


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Copyright information

© Humana Press 2010

Authors and Affiliations

  • Eric Gehrie
    • 1
  • William Van der Touw
    • 1
  • Jonathan S. Bromberg
    • 1
  • Jordi C. Ochando
    • 1
    • 2
    Email author
  1. 1.Department of Gene and Cell MedicineMount Sinai School of MedicineNew YorkUSA
  2. 2.Laboratorio de Immunología de TrasplantesCentro Nacional de Microbiología, Instituto de Salud Carlos IIIMadridSpain

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