Mass Spectrometric Protein Identification Using the Global Proteome Machine

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 673)

Abstract

Protein identification by mass spectrometry is widely used in biological research. Here, we describe how the global proteome machine (GPM) can be used for protein identification and for validation of the results. We cover identification by searching protein sequence collections and spectral libraries as well as validation of the results using expectation values, rho-diagrams, and spectrum databases.

Key words

Proteomics Mass spectrometry Protein identification Spectrum libraries Validation 

Notes

Acknowledgments

This work was supported by funding provided by the National Institutes of Health Grants RR00862 and RR022220, the Carl Trygger foundation, and the Swedish research council.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.The Rockefeller UniversityNew YorkUSA
  2. 2.Department of ChemistrySwedish University of Agricultural SciencesUppsalaSweden
  3. 3.The Biomedical Research CentreUniversity of British ColumbiaVancouverCanada

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