Computational Biology pp 189-202

Part of the Methods in Molecular Biology book series (MIMB, volume 673)

Mass Spectrometric Protein Identification Using the Global Proteome Machine

Protocol

Abstract

Protein identification by mass spectrometry is widely used in biological research. Here, we describe how the global proteome machine (GPM) can be used for protein identification and for validation of the results. We cover identification by searching protein sequence collections and spectral libraries as well as validation of the results using expectation values, rho-diagrams, and spectrum databases.

Key words

Proteomics Mass spectrometry Protein identification Spectrum libraries Validation 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.The Rockefeller UniversityNew YorkUSA
  2. 2.Department of ChemistrySwedish University of Agricultural SciencesUppsalaSweden
  3. 3.The Biomedical Research CentreUniversity of British ColumbiaVancouverCanada

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