Oral Biology pp 87-102

Part of the Methods in Molecular Biology book series (MIMB, volume 666)

Protocols to Study the Physiology of Oral Biofilms

  • José A. Lemos
  • Jacqueline Abranches
  • Hyun Koo
  • Robert E. Marquis
  • Robert A. Burne
Protocol

Abstract

The oral cavity harbors several hundred different bacterial species that colonize both hard (teeth) and soft tissues, forming complex populations known as microbial biofilms. It is widely accepted that the phenotypic characteristics of bacteria grown in biofilms are substantially different from those grown in suspensions. Because biofilms are the natural habitat for the great majority of oral bacteria, including those contributing to oral diseases, a better understanding of the physiology of adherent populations is clearly needed to control oral microbes in health and disease. In this chapter, we use oral streptococci as examples for studying the physiology of oral biofilms.

Key words

Biofilm oral streptococci Streptococcus enzymatic assays stress production of polysaccharides 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • José A. Lemos
    • 1
    • 2
  • Jacqueline Abranches
    • 1
    • 2
  • Hyun Koo
    • 3
    • 4
  • Robert E. Marquis
    • 3
  • Robert A. Burne
    • 5
  1. 1.Department of Microbiology and ImmunologyUniversity of Rochester Medical CenterRochesterUSA
  2. 2.Center for Oral Biology, University of Rochester Medical CenterRochesterUSA
  3. 3.Department of Microbiology and ImmunologyCenter for Oral Biology, University of Rochester Medical CenterRochesterUSA
  4. 4.Eastman Department of DentistryUniversity of Rochester Medical CenterRochesterUSA
  5. 5.Department of Oral BiologyUniversity of Florida College of DentistryGainesvilleUSA

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