HIV Drug Resistance Testing

Part of the Methods in Molecular Biology book series (MIMB, volume 665)


HIV genotypic resistance testing has become a routine test informing the management of HIV-positive patients. This chapter describes the background of this testing and provides a protocol for undertaking the assay by a home-brew method. Methods for interpretation of the data are also outlined.

Key words

HIV Drug resistance Genotypic interpretation HIV resistance testing Nucleotide sequencing Mutation interpretation 


  1. 1.
    Gazzard, B.G. (2008) BHIVA treatment guidelines writing group. British HIV association guidelines for the treatment of HIV-1-infected adults with antiretroviral therapy. HIV Med 9, 563–608.PubMedCrossRefGoogle Scholar
  2. 2.
  3. 3.
  4. 4.
  5. 5.
    Buckton, A.J., Bissett, S.L., Myers, R.E., Beddows, S., Cane, P.A., and Pillay, D. (2008) Development and optimization of an internally controlled dried blood spot assay for surveillance of human immunodeficiency virus type-1 drug resistance. J Antimicrob Chemother 62(6), 1191–1198.PubMedCrossRefGoogle Scholar
  6. 6.
    Shafer, R.W. and Schapiro, J.M. (2008) HIV-1 drug resistance mutations: an updated framework for the second decade of HAART. AIDS Rev 10, 67–84.PubMedGoogle Scholar
  7. 7.
  8. 8.
  9. 9.
    Cane, P.A., Kaye, S., Smit, E., Tilston, P., Kirk, S., Shepherd, J., Hopkins, M., Zhang, H., and Geretti, A.M. (2008) Genotypic antiretroviral drug resistance testing at low viral loads in the UK. HIV Med 9, 673–676.PubMedCrossRefGoogle Scholar
  10. 10.

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Virus Reference Department, Centre for InfectionsHealth Protection AgencyLondonUK

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