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Immunoelectron Microscopy of Cryofixed Freeze-Substituted Mammalian Tissue Culture Cells

  • Akira Sawaguchi
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 657)

Abstract

Mammalian tissue cultured cells are widely used in cell biology research. Immunoelectron microscopy is a powerful technique to define the subcellular localization of targeted antigens in the cultured cells. Cryofixation is now generally accepted as the best initial fixation step, to preserve the cellular fine structures and antigenicity. This chapter covers the practical procedures for immunoelectron microscopy of cryofixed freeze-substituted mammalian tissue cultured cells processed by high-pressure freezing.

Key words

Immunogold labeling cryofixation freeze-substitution mammalian cultured cells lowicryl resin potassium permanganate 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Akira Sawaguchi
    • 1
  1. 1.Department of Anatomy, Ultrastructural Cell Biology, Faculty of MedicineUniversity of MiyazakiMiyazakiJapan

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