Plant Developmental Biology pp 321-346

Part of the Methods in Molecular Biology book series (MIMB, volume 655) | Cite as

Laser Microdissection of Paraffin-Embedded Plant Tissues for Transcript Profiling

  • Robert C. Day
Protocol

Abstract

High-resolution cellular analysis will help answer many important questions in plant biology including how genetic information is differentially used to enable the formation and development of the plant body. By comparing transcriptome data from distinct cell types during various stages of development, insight can be obtained into the transcriptional networks that underpin the attributes and contributions of particular cells and tissues. Laser microdissection (LM) is a technique that enables researchers to obtain specific cells or tissues from histological samples in a manner conducive to downstream molecular analysis. LM has become an established strategy in many areas of biology and it has recently been adapted for use with many types of plant tissue.

Key words

Laser-capture microdissection laser microdissection gene expression paraffin sections chemical fixatives 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Robert C. Day
    • 1
  1. 1.Department of BiochemistryUniversity of OtagoDunedinNew Zealand

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