Laser Microdissection of Paraffin-Embedded Plant Tissues for Transcript Profiling
High-resolution cellular analysis will help answer many important questions in plant biology including how genetic information is differentially used to enable the formation and development of the plant body. By comparing transcriptome data from distinct cell types during various stages of development, insight can be obtained into the transcriptional networks that underpin the attributes and contributions of particular cells and tissues. Laser microdissection (LM) is a technique that enables researchers to obtain specific cells or tissues from histological samples in a manner conducive to downstream molecular analysis. LM has become an established strategy in many areas of biology and it has recently been adapted for use with many types of plant tissue.
Key wordsLaser-capture microdissection laser microdissection gene expression paraffin sections chemical fixatives
The authors would like to thank Bronwyn Carlisle for drawing Fig. 22.1. Protocols were developed in the Macknight laboratory with support from the Marsden Fund.
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