Mass Spectrometry Imaging pp 363-383

Part of the Methods in Molecular Biology book series (MIMB, volume 656)

Structurally Selective Imaging Mass Spectrometry by Imaging Ion Mobility-Mass Spectrometry

  • John A. McLean
  • Larissa S. Fenn
  • Jeffrey R. Enders

Abstract

This chapter describes the utility of structurally based separations combined with imaging mass spectrometry (MS) by ion mobility-MS (IM-MS) approaches. The unique capabilities of combining rapid (μs-ms) IM separations with imaging MS are detailed for an audience ranging from new to potential practitioners in IM-MS technology. Importantly, imaging IM-MS provides the ability to rapidly separate and elucidate various types of endogenous and exogenous biomolecules (e.g., nucleotides, carbohydrates, peptides, and lipids), including isobaric species. Drift tube and traveling wave IM-MS instrumentation are described and specific protocols are presented for calculating ion–neutral collision cross sections (i.e., apparent ion surface area or structure) from experimentally obtained IM-MS data. Special emphasis is placed on the use of imaging IM-MS for the analysis of samples in life sciences research (e.g., thin tissue sections), including selective imaging for peptide/protein and lipid distributions. Future directions for rapid and multiplexed imaging IM-MS/MS are detailed.

Key words

Ion mobility ion mobility-mass spectrometry IM-MS imaging mass spectrometry IMS MSI imaging ion mobility-mass spectrometry structural separations MALDI IM-MS/MS 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • John A. McLean
    • 1
  • Larissa S. Fenn
    • 1
  • Jeffrey R. Enders
    • 1
  1. 1.Department of ChemistryVanderbilt Institute of Chemical Biology, and Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt UniversityNashvilleUSA

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