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Alzheimer's Disease and Frontotemporal Dementia

Volume 670 of the series Methods in Molecular Biology pp 231-243

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Real-Time Visualization of Axonal Transport in Neurons

  • Yasuko OsakadaAffiliated withDepartment of Chemistry, Stanford University
  • , Bianxiao CuiAffiliated withDepartment of Chemistry, Stanford University Email author 

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Abstract

The normal function of neurons depends on the integrity of microtubule-dependent transport of cellular materials and organelles to/from their cell bodies or axon terminus. In this chapter, we describe the design and implementation of a fluorescence imaging method to visualize axonal transport in neurons directly. We combine a pseudo total internal reflection microscopy, quantum dot fluorescence labeling, microfluidic neuronal culture chamber, and single molecule detection methods to achieve a high spatial and temporal resolution in tracking nerve growth factor transport in dorsal root ganglia neurons.

Key words

Axonal transport Nerve growth factor Live imaging Quantum dot Microfluidic Total internal reflection microscopy Single molecule imaging