Surface Patterning for Generating Defined Nanoscale Matrices

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 660)

Abstract

While stem cells in culture have been predominately controlled through the addition of soluble factors to the media, the impact of the extracellular matrix on stem cell renewal and differentiation has recently come to the forefront. In vivo, cells adhere and respond to cues that are on the nanoscale, thus the presentation of extracellular matrix components on this scale is critical to mimicking the in vivo environment. We have developed a highly flexible nanopatterning technique, employing protein and peptide reactive polymers and electron beam lithography, which can be utilized for studying matrix effects on stem cell renewal and differentiation.

Key words

Extracellular matrix Patterning Nanotechnology Stem cells Differentiation 

Notes

Acknowledgments

The authors would like to thank Eric Schopf and Kevin Chung in helping to develop these techniques. This work was supported by the NIH NIBIB (R21 EB 005838, HDM), an NIH NHLBI Postodoctoral Fellowship (5F32HL082138-02, KLC), and the NIH Director’s New Innovator Award Program, part of the NIH Roadmap for Medical Research, through grant number 1-DP2-OD004309-01 (KLC).

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of BioengineeringUniversity of CaliforniaSan DiegoUSA
  2. 2.Department of Chemistry & BiochemistryUniversity of CaliforniaLos AngelesUSA

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