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An Array Platform for Identification of Stress-Responsive MicroRNAs in Plants

  • Xiaoyun Jia
  • Venugopal Mendu
  • Guiliang Tang
Part of the Methods in Molecular Biology book series (MIMB, volume 639)

Abstract

MicroRNAs (miRNAs) are approximately 22-nucleotide (nt)-long non-coding RNAs that play a key role in plant development and abiotic stresses. We have developed a simple but effective array platform for profiling plant miRNAs from various plant species. The array is composed of 188 non-redundant miRNA probes that can detect both conserved and species-specific miRNAs from most plant species, including Arabidopsis, rice, and poplar. In this chapter, we describe a protocol for developing the miRNA array platform, which can be used to identify stress-responsive miRNAs in diverse plant species. Profiling of miRNAs in tobacco seedlings exposed to different abiotic stress conditions has revealed that a number of miRNAs, miR398, miR399, miR408, miR156, miR164, and miR168, were responsive to stresses. This comprehensive and easy-to-follow protocol will be useful for studying roles of miRNAs in plant stress response.

Key words

Abiotic stress microRNAs microRNA array small RNA blot analysis 

Notes

Acknowledgments

G.T. is supported by the Kentucky Tobacco Research and Development Center (KTRDC), the USDA-NRI grants 2006-35301-17115 and 2006-35100-17433, the NSF MCB-0718029 (Subaward No. S-00000260), and an award from the Kentucky Science and Technology Corporation under Contract # KSTC-144-401-08-029.

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Copyright information

© Humana Press 2010

Authors and Affiliations

  • Xiaoyun Jia
    • 1
  • Venugopal Mendu
    • 1
  • Guiliang Tang
    • 1
  1. 1.Department of Plant and Soil Sciences and KTRDCUniversity of KentuckyLexingtonUSA

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