Photodynamic Therapy and Cell Death Pathways

  • David Kessel
  • Nancy L. Oleinick
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 635)

Abstract

Photodynamic therapy (PDT) is the term used to describe the irradiation of photosensitized cells or tissue with phototoxic consequences. This process can result in the rapid initiation of not only apoptosis, an irreversible death pathway, but also autophagy. The procedures described here are designed to characterize the correlation between the PDT dose vs. survival of cells in vitro, the apoptotic effects of photodamage, and the extent of an autophagic response. These are assessed by clonogenic assays, observation of condensed chromatin characteristic of apoptosis, activation of “executioner” caspases, and the autophagic flux as indicated by comparing accumulation of the LC3-II protein under conditions where processing of autophagosomes is retarded vs. is not retarded.

Key words

Apoptosis autophagy Bcl-2 Bid Bax photosensitization photodynamic therapy 

Notes

Acknowledgments

The authors’ research is supported by NIH grant CA 23378 (to DK) and NIH grant CA 106491 (to NLO) from the National Cancer Institute, DHHS and by the State of Ohio Biomedical Research and Technology Transfer Trust TECH 05-063 (to NLO).

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • David Kessel
    • 1
  • Nancy L. Oleinick
    • 2
  1. 1.Wayne State University School of MedicineDetroitUSA
  2. 2.Case Western Reserve UniversityClevelandUSA

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