Cellular Programming and Reprogramming pp 285-292

Part of the Methods in Molecular Biology book series (MIMB, volume 636)

In Vitro Reprogramming of Pancreatic Cells to Hepatocytes

  • Daniel Eberhard
  • Kathy O’Neill
  • Zoë D. Burke
  • David Tosh
Protocol

Abstract

Transdifferentiation is defined as the conversion of one cell type to another. One well-documented example of transdifferentiation is the conversion of pancreatic cells to hepatocytes. Here we describe a robust in vitro model to study pancreas to liver transdifferentiation. It is based on the addition of the synthetic glucocorticoid dexamethasone to the rat pancreatic exocrine cell line AR42J. Following glucocorticoid treatment, cells resembling hepatocytes are induced. Transdifferentiated hepatocytes express many of the properties of bona fide hepatocytes, e.g. production of albumin and ability to respond to xenobiotics. These hepatocytes can be used for studying liver function in vitro as well as studying the molecular basis of transdifferentiation.

Key words

Transdifferentiation AR42J Exocrine pancreas Hepatocytes Dexamethasone Oncostatin M 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Daniel Eberhard
    • 1
  • Kathy O’Neill
    • 1
  • Zoë D. Burke
    • 1
  • David Tosh
    • 1
  1. 1.Department of Biology & BiochemistryCentre for Regenerative Medicine, University of BathBathUK

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