RNA Therapeutics pp 237-253

Part of the Methods in Molecular Biology book series (MIMB, volume 629)

Inhibition of the microRNA Pathway in Zebrafish by siRNA

  • Anders Fjose
  • Xiao-Feng Zhao


The microRNA (miRNA) pathway and the phenomenon of RNA interference (RNAi), which have both been shown to involve targeting of mRNAs by small RNA molecules, are interconnected and depend partly on the same cellular machinery. RNAi in vertebrates was first reported in zebrafish (Danio rerio) 10 years ago. However, reliable RNAi-based gene silencing techniques, based on injection of small interfering RNAs (siRNAs) into zygotes, have not been established for this important vertebrate model because of unspecific developmental defects. We have recently shown that these side effects can be attributed to inhibition of the miRNA pathway by siRNAs at early embryonic stages. This review highlights these findings and the function of microRNAs in zebrafish development.

Key words

RNAi siRNA microRNA miR-430 zebrafish 


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Anders Fjose
    • 1
  • Xiao-Feng Zhao
    • 1
  1. 1.Department of Molecular BiologyUniversity of BergenBergenNorway

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