Functional Studies on RNA-Transfected Cell Microarrays
RNA-transfected cell microarray shows great promise in functional genomics. By printing siRNA complexed with transfection reagent on glass slides, arrays of transfected cells are formed in which the phenotypic consequences of gene suppression can be investigated. Using reporter plasmids with fluorescence intensity as output data, we have developed a strategy for statistical analysis of the intensity data from medium-scale functional studies using data from several experimental replicates.
Key wordsReverse transfection RNA interference small interfering RNA (siRNA) linear regression (ANOVA) modeling
We thank Hallgeir Bergum and Atle van Beelen Granlund at the National Technology Microarray Platform (Norwegian Microarray Consortium) at NTNU, which is founded by the Functional Genomics Programme (FUGE) of the Norwegian Research Counsil for valuable input and help in the work of establishing transfected cell microarrays in our lab. Funding was provided by Liaison Committee between the Central Norwegian Region Health Authority (RHA) and NTNU.
- 16.Hu, Y.H., Warnatz, H.J., Vanhecke, D., Wagner, F., Fiebitz, A., Thamm, S., Kahlem, P., Lehrach, H., Yaspo, M.L., and Janitz, M. (2006) Cell array-based intracellular localization screening reveals novel functional features of human chromosome 21 proteins. BMC Genomics, 7, 155.PubMedCrossRefGoogle Scholar