Quantitative Neuroproteomics of the Synapse
An emerging way to study neuropsychiatric or neurodegenerative diseases is by performing proteomic analyses of brain tissues. Here, we describe methods used to isolate and identify the proteins associated with a sample of interest, such as the synapse, as well as to compare the levels of proteins in the sample under different conditions. These techniques, involving subcellular fractionation and modern quantitative proteomics using isotopic labels, can be used to understand the organization of neuronal compartments and the regulation of synaptic function under various conditions.
Key wordsNeuroproteomics subcellular fractionation presynaptic terminal mass spectrometry quantitative proteomics differential isotopic labelling
This work is partially supported by the National Institute on Drug Abuse through Awards No. DA018310 and DA017940 to JVS.
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