Peptidomics pp 227-246 | Cite as

Quantitative Neuroproteomics of the Synapse

  • Dinah Lee Ramos-Ortolaza
  • Ittai Bushlin
  • Noura Abul-Husn
  • Suresh P. Annangudi
  • Jonathan Sweedler
  • Lakshmi A. Devi
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 615)

Abstract

An emerging way to study neuropsychiatric or neurodegenerative diseases is by performing proteomic analyses of brain tissues. Here, we describe methods used to isolate and identify the proteins associated with a sample of interest, such as the synapse, as well as to compare the levels of proteins in the sample under different conditions. These techniques, involving subcellular fractionation and modern quantitative proteomics using isotopic labels, can be used to understand the organization of neuronal compartments and the regulation of synaptic function under various conditions.

Key words

Neuroproteomics subcellular fractionation presynaptic terminal mass spectrometry quantitative proteomics differential isotopic labelling 

Notes

Acknowledgements

This work is partially supported by the National Institute on Drug Abuse through Awards No. DA018310 and DA017940 to JVS.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Dinah Lee Ramos-Ortolaza
    • 1
  • Ittai Bushlin
    • 1
  • Noura Abul-Husn
    • 1
  • Suresh P. Annangudi
    • 2
  • Jonathan Sweedler
    • 2
  • Lakshmi A. Devi
    • 1
  1. 1.Department of Pharmacology and Systems TherapeuticsMount Sinai School of MedicineNew YorkUSA
  2. 2.Department of Chemistry and the Beckman InstituteUniversity of Illinois at Urbana-ChampaignUrbanaUSA

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