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Advanced Protocols in Oxidative Stress II pp 275–285Cite as

LIBS-Based Detection of Antioxidant Elements: A New Strategy

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 594))

Abstract

The present study deals with the scientific evaluation of antioxidant potential of aqueous extract of Trichosanthes dioica fruits on diabetes-induced oxidative stress of diabetic rats. The most effective dose of mg/kg bw of fruit aqueous extract was given orally to diabetic rats for 30 days. Different oxidative stress parameters were analyzed in various tissues of control and treated diabetic rats. The observed elevated level of lipid peroxidation (LPO) comes down significantly (p < 0.05) and decreased activities of antioxidant enzymes such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) got increased (p < 0.05) significantly of diabetic rats on extract treatment. Laser-Induced Breakdown Spectroscopy (LIBS) has been used as an analytical tool to detect major and minor elements like Mg, Fe, Na, K, Zn, Ca, H, O, C, and N present in the extract. The higher concentration of Ca2+, Mg2+, and Fe2+, as reflected by their intensities are responsible for antioxidant potential of T. dioica.

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Acknowledgements

The authors are grateful to DRDO, National Medicinal Plants board (NMPB) New Delhi, India, for providing the financial assistance. PKR and DKR are thankful to Indian Council of Medical Research (ICMR) and Council of Scientific and Industrial Research (CSIR) respectively, for the award of SRF to them. The drawing of the experimental setup by Mr. Vivek Kumar Singh, Department of Physics, University of Allahabad is acknowledged.

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Authors and Affiliations

  1. Department of Biochemistry, University of Allahabad, Allahabad, India

    Geeta Watal, Prashant Kumar Rai & Dolly Jaiswal

  2. Medicinal Research Lab, Drug Discovery & Development Division, Alternative Therapeutics Unit, Department of Chemistry, University of Allahabad, Allahabad, India

    Bechan Sharma & Devendra K. Rai

  3. Laser Spectroscopy Research Laboratory, Department of Physics, University of Allahabad, Allahabad, India

    Nilesh K. Rai & A. K. Rai

Authors
  1. Geeta Watal
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  2. Bechan Sharma
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  3. Prashant Kumar Rai
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  4. Dolly Jaiswal
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  5. Devendra K. Rai
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  6. Nilesh K. Rai
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  7. A. K. Rai
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Corresponding author

Correspondence to Geeta Watal .

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Editors and Affiliations

  1. Golden Leaf Rd. 215, Mars Hill, 28754, USA

    Donald Armstrong

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© 2010 Humana Press, a part of Springer Science+Business Media, LLC

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Watal, G. et al. (2010). LIBS-Based Detection of Antioxidant Elements: A New Strategy. In: Armstrong, D. (eds) Advanced Protocols in Oxidative Stress II. Methods in Molecular Biology, vol 594. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-411-1_19

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  • DOI: https://doi.org/10.1007/978-1-60761-411-1_19

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-410-4

  • Online ISBN: 978-1-60761-411-1

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