Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine Conditions

  • Frédéric Minner
  • Françoise Herphelin
  • Yves Poumay
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 585)

Abstract

This chapter deals with protocols to set up human keratinocyte cultures in serum-free conditions and lead them to autocrine autonomously growing conditions. These conditions have proven adequate for studies of epidermal differentiation by measurements of the expression of typical early and late differentiation markers. The chapter also deals with the use of quantitative RT-PCR in order to determine the epidermal marker gene expression levels by comparison with adequate housekeeping genes.

Key words

Keratinocytes Autocrine culture Cell density Epidermal differentiation markers Quantitative RT-PCR 
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Notes

Acknowledgments

This work was financially supported by FRFC grant 2.4506.01 and FNRS grant 1.5.033.06F to YP.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Frédéric Minner
    • 1
  • Françoise Herphelin
    • 1
  • Yves Poumay
    • 1
  1. 1.Cell and Tissue LaboratoryURPHYM, University of Namur (FUNDP)NamurBelgium

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