Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization

  • Maria I. Morasso
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 585)

Abstract

Although recent molecular biology advances provide a very effective way in determining localized gene expression, the visualization of the expression by in situ hybridization of whole-mount embryos or paraffin-embedded tissue sections continues to be an excellent method to determine overall gene expression. In this chapter, I review protocols designed to determine mRNA expression by in situ hybridization techniques, in particular, focusing on the developing stratified epidermis.

Key words

DNA RNA Gene expression Epidermis In situ hybridization 

References

  1. 1.
    Morasso, M.I., Grinberg, A., Robinson, G., Sargent, T.D. and Mahon, K.A. (1999) Placental failure in mice lacking the homeobox gene Dlx3. Proc. Nat. Acad. Sci. USA. 96, 162–167.PubMedCrossRefGoogle Scholar
  2. 2.
    Park, G.-T., Lim, S., Jang, S. and Morasso, M.I. (2002) Suprabasin, a novel epidermal differentiation marker and potential cornified envelope precursor. J. Biol. Chem.277, 45195–45202.PubMedCrossRefGoogle Scholar
  3. 3.
    Hwang, M. and Morasso, M.I. (2003) The novel murine Ca++-binding protein Scarf is differentially expressed during epidermal differentiation. J. Biol. Chem. 278, 47827–47833.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Maria I. Morasso
    • 1
  1. 1.Developmental Skin Biology Section, NIH/NIAMSBethesdaUSA

Personalised recommendations