Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization

  • Maria I. Morasso
Part of the Methods in Molecular Biology book series (MIMB, volume 585)


Although recent molecular biology advances provide a very effective way in determining localized gene expression, the visualization of the expression by in situ hybridization of whole-mount embryos or paraffin-embedded tissue sections continues to be an excellent method to determine overall gene expression. In this chapter, I review protocols designed to determine mRNA expression by in situ hybridization techniques, in particular, focusing on the developing stratified epidermis.

Key words

DNA RNA Gene expression Epidermis In situ hybridization 


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    Hwang, M. and Morasso, M.I. (2003) The novel murine Ca++-binding protein Scarf is differentially expressed during epidermal differentiation. J. Biol. Chem. 278, 47827–47833.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Maria I. Morasso
    • 1
  1. 1.Developmental Skin Biology Section, NIH/NIAMSBethesdaUSA

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