Cytoskeleton Methods and Protocols pp 41-68

Part of the Methods in Molecular Biology book series (MIMB, volume 586)

Live-Cell Imaging of the Cytoskeleton and Mitochondrial–Cytoskeletal Interactions in Budding Yeast

  • Theresa C. Swayne
  • Thomas G. Lipkin
  • Liza A. Pon
Protocol

Summary

This chapter describes labeling methods and optical approaches for live-cell imaging of the cytoskeleton and of a specific organelle–cytoskeleton interaction in budding yeast.

Key words

Yeast Mitochondria Fluorescent proteins Immunofluorescence Vital staining Microscopy Live-cell imaging Deconvolution 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Theresa C. Swayne
    • 1
  • Thomas G. Lipkin
    • 2
  • Liza A. Pon
    • 1
  1. 1.Department of Pathology and Cell Biology, College of Physicians and SurgeonsColumbia UniversityNew YorkUSA
  2. 2.Department of MCD BiologyUniversity of ColoradoBoulderUSA

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