Study of Gap Junctions in Human Embryonic Stem Cells

  • Raymond C.B. Wong
  • Alice Pébay
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 584)

Abstract

Gap junctional intercellular communication (GJIC) has been described in different cell types including stem cells and has been involved in different biological events. GJIC is required for mouse embryonic stem cell maintenance and proliferation, and various studies suggest that functional GJIC is a common characteristic of human embryonic stem cells (hESC) maintained in different culture conditions. This chapter introduces methods to study gap junctions in hESC, from expression of gap junction proteins to functional study of GJIC in hESC proliferation, apoptosis, colony growth, and pluripotency.

Key words

Connexin gap junction gap junctional intercellular communication human embryonic stem cells 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2006 2009

Authors and Affiliations

  • Raymond C.B. Wong
    • 1
  • Alice Pébay
    • 2
  1. 1.Department of Biological ChemistryUniversity of California IrvineIrvineUSA
  2. 2.Centre for Neuroscience and Department of PharmacologyThe University of MelbourneParkvilleAustralia

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