Natural Killer Cell Protocols pp 127-148

Part of the Methods in Molecular Biology book series (MIMB, volume 612) | Cite as

Analysis of the NK Cell Immunological Synapse

  • Keri B. Sanborn
  • Gregory D. Rak
  • Ashley N. Mentlik
  • Pinaki P. Banerjee
  • Jordan S. Orange
Protocol

Abstract

Since NK cells specialize in contact-dependent functions including cytotoxicity, interest has focused on the direct study of the interface between the NK cell and the cell with which it is interacting. This interface is also known as the immunological synapse and is characterized by an extraordinary number of dynamic molecular events that have the potential to result in NK cell function. Here we describe microscopy-based methods for evaluating and quantifying the NK cell immunological synapse that can be useful in enabling experimental studies.

Key words

Immunological synapse confocal microscopy colocalization fluorescence 

References

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Keri B. Sanborn
    • 1
  • Gregory D. Rak
    • 2
  • Ashley N. Mentlik
    • 3
  • Pinaki P. Banerjee
    • 4
  • Jordan S. Orange
    • 5
  1. 1.Immunology Graduate Group, Division of ImmunologyChildren’s Hospital of Philadelphia, University of Pennsylvania School of MedicinePhiladelphiaUSA
  2. 2.Cell Biology and Physiology Graduate Group, Division of ImmunologyChildren’s Hospital of Philadelphia, University of Pennsylvania School of Medicine and School of Veterinary MedicinePhiladelphiaUSA
  3. 3.Cell Biology and Physiology Graduate Group, Division of ImmunologyChildren’s Hospital of Philadelphia, University of Pennsylvania School of MedicinePhiladelphiaUSA
  4. 4.Division of ImmunologyChildren’s Hospital of PhiladelphiaPhiladelphiaUSA
  5. 5.Division of ImmunologyThe Joseph Stokes Jr. Research Institute, Children’s Hospital of Philadelphia, University of Pennsylvania School of Medicine and School of Veterinary MedicinePhiladelphiaUSA

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