Natural Killer Cell Protocols pp 365-375

Part of the Methods in Molecular Biology book series (MIMB, volume 612)

KIR Genotyping by Multiplex PCR-SSP

  • Smita Kulkarni
  • Maureen P. Martin
  • Mary Carrington
Protocol

Abstract

Diversity across KIR haplotypes stems from differences in numbers of inhibitory and activating receptors, as well as allelic polymorphism of individual genes. The KIR locus has undergone large expansions and contractions over time and is believed to be coevolving with genes encoding its HLA class I ligands located within the MHC locus. KIR and HLA compound genotypes have been associated with susceptibility to or protection from infectious, autoimmune, reproductive, and malignant disorders. We describe here a simple and reliable multiplex PCR-SSP (sequence-specific priming) method for relatively rapid and inexpensive genotyping of 15 KIR genes using standard agarose gel electrophoresis.

Key words

KIR genotyping NK cell KIR haplotypes multiplex PCR PCR-SSP 

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Smita Kulkarni
    • 1
  • Maureen P. Martin
    • 1
  • Mary Carrington
    • 1
  1. 1.Cancer and Inflammation Program, Laboratory of Experimental ImmunologySAIC-Frederick Inc., National Cancer Institute-FrederickFrederickUSA

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