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Membrane Protein Solubilization

  • Katia Duquesne
  • James N. SturgisEmail author
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 601)

Abstract

A critical step in any in vitro analysis of membrane proteins is the solubilization of the membrane to extract the protein of interest in an active form to obtain an aqueous solution containing the membrane protein complexed with detergents and lipids in a form suitable for purification and further analysis. This process is particularly delicate as the aim is to maximally disrupt the lipid components of the membrane while putting the protein components in an un-natural detergent environment without perturbing them. Looked at this way, it is remarkable that it ever works. Although the process is difficult and hard to master, an increasing number of membrane proteins have been successfully solubilized in active forms, allowing some general principles to be established that we illustrate in the method developed in this chapter.

Key words

Cytoplasmic membrane detergent membrane proteins purification titration 

Notes

Acknowledgements

This work, and K.D., were supported by the CNRS and ANR (grant number Blanc-2007-1_183779).

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Laboratoire d’Ingenierie des Systèmes MaromoléculaireInstitut de Biologie Structurale et Microbiologie, CNRS and Aix Marseille UniversitéMarseilleFrance

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