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Lipidomics pp 391-411 | Cite as

Membrane Lipidomics and the Geometry of Unsaturated Fatty Acids From Biomimetic Models to Biological Consequences

  • Carla Ferreri
  • Chryssostomos Chatgilialoglu
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 579)

Summary

In the last decades, free radical processes delineated an interdisciplinary field linking chemistry to biology and medicine. Free radical mechanisms became of importance as molecular basis of physiological and pathological conditions. Lipids, in particular, unsaturated fatty acids, are susceptible of free radical attack. The reactivity of the double bond toward free radicals is well known, in particular the reversible addition of radical species to this functionality determines the cistrans double bond isomerization. Since the prevalent geometry displayed by unsaturated fatty acids in eukaryotes is cis, the occurrence of the cistrans isomerization by free radicals corresponds to the loss of an important structural information linked to biological activity. The formation of trans isomers can have important meaning and consequences connected to radical stress.

Free radical isomerization of membrane fatty acids has been the subject of research coupling the top-down approach by model studies, such as biomimetic chemistry in liposomes, with the bottom-up approach dealing with the examination of cell membrane lipidome in living systems under several physiopathological conditions. Methodologies and molecular libraries have been settled, for both liposome experiments and the examination of the radical stress in biological membranes. This chapter will give an overview of the current procedures used for liposome models and the cistrans isomerization experiments, in order to build-up a library of trans geometrical fatty acid isomers.

Key words

Membrane lipidomics Geometrical trans fatty acid Radical stress Liposome Biomimetic chemistry Free radical isomerization 

Notes

Acknowledgments

The authors wish to thank all collaborators that helped to develop methodologies in an interdisciplinary context. Support and sponsorship of the COST Action CM0603 “Free Radicals in Chemical Biology” is gratefully acknowledged.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Carla Ferreri
    • 1
  • Chryssostomos Chatgilialoglu
    • 2
  1. 1.ISOF-BioFree Radicals, Consiglio Nazionale delle RicericheBolognaItaly
  2. 2.ISOF, Consiglio Nazionale delle RicercheBolognaItaly

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