Reverse Chemical Genetics pp 3-9

Part of the Methods in Molecular Biology™ book series (MIMB, volume 577)

ORFeome Cloning

  • Osamu Ohara
Protocol

Summary

Reverse chemical genomic approach is expected to greatly expedite the discovery of new compounds, which modulate biological phenotypes in various ways. However, toward this end, various contents and platforms must be well prepared in the research community. In this regard, genome-wide preparation of clones for production of proteins in either a native or a fusion form, which are conventionally called ORFeome clones, would play a crucial role in realizing reverse chemical genomics as an approach of choice. In this chapter, currently available ORFeome cloning technologies are overviewed and a selection guideline for them is provided.

Key words

ORFeome cloning Protein production Recombination Fusion protein 

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Osamu Ohara
    • 1
    • 2
  1. 1.Department of Human Genome ResearchKazusa DNA Research InstituteChibaJapan
  2. 2.Laboratory for ImmunogenomicsRIKEN Research Institute for Allergy and ImmunologyYokohama CityJapan

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