Skip to main content
Book cover

Biofuels pp 247–261Cite as

Basic Laboratory Culture Methods for Anaerobic Bacteria

Part of the Methods in Molecular Biology book series (MIMB,volume 581)

Summary

Oxygen is either limiting or absent in many ecosystems. Anaerobic bacteria are often key players in such environments and these organisms have important roles in geo-elemental cycling, agriculture, and medicine. The metabolic versatility of anaerobes is exploited in a variety of industrial processes including fermented food production, biochemical synthesis, and bioremediation. There has been recent considerable interest in developing and enhancing technologies that employ anaerobes as biocatalysts. The study of anaerobic bacteria requires specialized techniques, and specific methods are described for the culture and manipulation of these microbes.

Key words

  • Anaerobic bacteria
  • Batch culture
  • Continuous culture

This is a preview of subscription content, access via your institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-1-60761-214-8_16
  • Chapter length: 15 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   119.00
Price excludes VAT (USA)
  • ISBN: 978-1-60761-214-8
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   159.99
Price excludes VAT (USA)
Hardcover Book
USD   159.99
Price excludes VAT (USA)
Learn about institutional subscriptions
Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Hungate, R. E. (1969) A roll tube method for cultivation of strict anaerobes, in Methods in Microbiology, Vol. 3b (Norris, J. R. and Ribbons, D. W., ed.), Academic, New York, pp. 117–132.

    Google Scholar 

  2. Bryant, M. P (1972) Commentary on the Hungate technique for culture of anaerobic bacteria. Am. J. Clin. Nutr. 25, 1324–1328.

    CAS  Google Scholar 

  3. Balch, W. E., Fox, G. E., Magrum, L. J., Woese, C. R., and Wolfe, R. S. (1979) Methanogens: reevaluation of a unique biological group. Microbiol. Mol. Biol. Rev. 43, 260–296.

    CAS  Google Scholar 

  4. Hungate, R. E. (1950) The anaerobic mesophilic cellulolytic bacteria. Bacteriol. Rev. 14, 1–49.

    CAS  Google Scholar 

  5. Monod, J. (1950) La technique de culture continue, theorie et applications. Ann. Inst. Pasteur 79, 390–410.

    CAS  Google Scholar 

  6. Novick, A. and Szilard, L (1950) Description of the chemostat. Science 112, 715–716.

    CrossRef  CAS  Google Scholar 

  7. Herbert, D. R., Elsworth, R., and Telling R. C. (1956) The continuous culture of bacteria: a theoretical and experimental study. J. Gen. Microbiol. 14, 601–622.

    CrossRef  CAS  Google Scholar 

  8. Pirt, S. J. (1975) Principles of Microbe and Cell Cultivation. Blackwell, Oxford.

    Google Scholar 

  9. Kistner, A. and Kornelius, J. H. (1990) A small-scale, 3-vessel, continuous culture system for quantitative studies of plant fiber degradation by anaeraobic0bacteria. J. Microbiol. Meth. 12, 173–182.

    CrossRef  Google Scholar 

  10. Pavlostathis, S. G., Miller, T. L., and Wolin, M. J. (1988) Fermentation of insoluble cellulose by continuous cultures of Ruminococcus albus. Appl. Environ. Microbiol. 54, 2655–2659.

    CAS  Google Scholar 

  11. Fukushima, R. S., Weimer, P. J., and Kunz, D. A. (2002) Photocatalytic interaction of resazurin N-oxide with cysteine optimizes preparation of anaerobic culture media. Anaerobe 8, 29–34.

    CrossRef  CAS  Google Scholar 

  12. Jones, G. A. and Pickard, M. D. (1980) Effect of titanium (III) citrate as reducing agent on growth of rumen bacteria. Appl. Environ. Microbiol. 39, 1144–1147.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Animal and Food Sciences, University of Kentucky, Lexington, KY, USA

    Herbert J. Strobel

Authors
  1. Herbert J. Strobel
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Bioconversion Science & Technology, Oak Ridge National Laboratory, Bethel Valley Road 1, Oak Ridge, 37831-6341, U.S.A.

    Jonathan R. Mielenz

Rights and permissions

Reprints and Permissions

Copyright information

© 2009 Humana Press, a part of Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Strobel, H.J. (2009). Basic Laboratory Culture Methods for Anaerobic Bacteria. In: Mielenz, J. (eds) Biofuels. Methods in Molecular Biology, vol 581. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-214-8_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-60761-214-8_16

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60761-213-1

  • Online ISBN: 978-1-60761-214-8

  • eBook Packages: Springer Protocols