Protocol

Proteomics

Volume 564 of the series Methods in Molecular Biology™ pp 105-121

Date:

Capillary Electrophoresis Coupled to Mass Spectrometry for Proteomic Profiling of Human Urine and Biomarker Discovery

  • Petra ZürbigAffiliated withMosaiques diagnostics GmbH Email author 
  • , Eric SchifferAffiliated withMosaiques diagnostics GmbH
  • , Harald MischakAffiliated withMosaiques diagnostics GmbH

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Summary

Currently, the main focus of clinical proteome analysis is on detection and identification of polypeptides that significantly change owing to pathological changes. Capillary electrophoresis coupled online to an electrospray ionization time of flight mass spectrometer (CE-MS) allows the differential display of a large number of polypeptides in a single, reproducible, and time-limited step and enables the comparison of different protein profiles for biomarker discovery. In addition to the reproducibility of the CE-MS setup, many further steps including data processing and mining, usage of biomarkers for diagnosis, and biomarker sequencing are necessary to answer the demands of biomarker discovery of clinical significance. In this chapter, we discuss materials and methods for CE-MS-based clinical proteomics allowing the reproducible profiling of urine.

Key words

Capillary electrophoresis Mass spectrometry Urine Polypeptides Biomarker discovery