Protocol

Meiosis

Volume 558 of the series Methods in Molecular Biology pp 131-145

Date:

Cytological Analysis of Arabidopsis thaliana Meiotic Chromosomes

  • Susan J. ArmstrongAffiliated withSchool of Biosciences, University of Birmingham
  • , Eugenio Sanchez-MoranAffiliated withSchool of Biosciences, University of Birmingham
  • , F. ChrisAffiliated withSchool of Biosciences, University of Birmingham
  • , H. FranklinAffiliated withSchool of Biosciences, University of Birmingham

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Abstract

Advances in molecular biology and in the genetics of Arabidopsis thaliana have led to this organism becoming an important model for the analysis of meiosis in plants. Cytogenetic investigations are pivotal to meiotic studies and a number of technological improvements for Arabidopsis cytology have provided a range of tools to investigate chromosome behaviour during meiosis. This chapter includes protocols on basic cytology, FISH analysis, immunocytology, a procedure for a meiotic time course and electron microscopy.

Key words

Arabidopsis thaliana meiosis cytogenetics FISH immunocytology meiotic time course electron microscopy